cfx 96 instrument (Bio-Rad)
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cfx 96 instrument
Cfx 96 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 20032 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx+96+instrument/CFX96+Touch/pm41391609-49-6-9
Average 99 stars, based on 20032 article reviews
Cfx 96 Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 20032 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfx+96+instrument/CFX96+Touch/pm41391609-49-6-9
Average 99 stars, based on 20032 article reviews
cfx 96 instrument - by Bioz Stars,
2026-09
99/100 stars
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Real-time Polymerase Chain Reaction:Article Title: First Report of Streptococcus agalactiae Meningitis in a Non-Pregnant Adult in Italy Article Snippet: The reaction mixture was prepared in a final volume of 25 μL, including 1 μM of each primer, 0.5 μM of the probe, TaqMan Universal PCR Master Mix 1X (Applied Biosystems, Waltham, MA, USA) and 5 μL of DNA template. .. Thermocycling conditions consisted of an initial denaturation step at 95 °C for 10 min, followed by 45 cycles at 94 °C for 30 s and 60 °C for 1 min. Real-time PCR was carried out on a Article Title: Near-freezing temperature alters ROS metabolism and gene transcription to enhance peach chilling tolerance and aroma quality Article Snippet: The effect of near-freezing temperature (NFT, − 1.1 C ± 0.1 C) on reducing chilling injury (CI) and flavor loss in peaches was systematically evaluated.. NFT effectively suppressed the incidence of internal browning (IB) in peach flesh, enabling for the maintenance of a normal ripening process after cold storage.. Concurrently, NFT significantly diminished the malondialdehyde (MDA) level and facilitated the accumulation of ascorbic acid (AsA) and glutathione (GSH). Article Title: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21. Article Snippet: The membranes were then developed using SuperSignalTM West Pico PLUS ECL Substrate (Thermo Fisher Scientific 34,580). .. Scientific Reports | (2025) 15:22311 3| https://doi.org/10.1038/s41598-025-06348-4 RNA extraction, reverse-transcription and quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cells with RNAiso Plus (Takara 9109) reagent and reverse-transcribed to cDNA with ALL-in-One First-Strand Synthesis MasterMix (with dsDNase). qRT-PCR was performed using SYBR GREEN (Yeasen 11201ES08) according to the manufacturer’s protocol with Article Title: Rapid and sensitive detection of
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tropical race 4 using a RPA-DETECTR assay Article Snippet: Sensitivity was further assessed using qPCR using AF primers (Table 1), qPCR amplification of DETECTR target sequences was carried out in 20 μl reaction volume using Foc TR4 specific AF primer pair, 10 μl of 2 × Sso Advanced Universal SYBR Green Supermix (Bio-Rad) and 1.0 μl of genomic DNA template, 0.3 μl of 10 μM of forward and reverse primers with volume makeup (20 μl) with the addition of RNase-free water. .. The qPCR program was followed as initial denaturation at 98°C for 5 min, followed by 40 cycles of denaturation at 95°C for 15 s, annealing and extension at 62°C for 30 s, followed by a melt curve analysis (60°C–95°C: increment 0.5°C per every 5 s). qPCR was conducted on a Article Title: S-palmitoylation of MTDH regulates ferroptosis resistance in breast cancer cell. Article Snippet: Reverse transcription PCR was performed using Hifair® AdvanceFast 1st Strand cDNA Synthesis Kit (Yeasen, 11150ES60) to obtain cDNA as template for quantitative RT-PCR. .. The quantitative RT-PCR was performed using Hieff UNICON® Advanced qPCR SYBR Master Mix (Yeasen, 11185ES03) in the RNA Extraction:Article Title: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21. Article Snippet: The membranes were then developed using SuperSignalTM West Pico PLUS ECL Substrate (Thermo Fisher Scientific 34,580). .. Scientific Reports | (2025) 15:22311 3| https://doi.org/10.1038/s41598-025-06348-4 RNA extraction, reverse-transcription and quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cells with RNAiso Plus (Takara 9109) reagent and reverse-transcribed to cDNA with ALL-in-One First-Strand Synthesis MasterMix (with dsDNase). qRT-PCR was performed using SYBR GREEN (Yeasen 11201ES08) according to the manufacturer’s protocol with Reverse Transcription:Article Title: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21. Article Snippet: The membranes were then developed using SuperSignalTM West Pico PLUS ECL Substrate (Thermo Fisher Scientific 34,580). .. Scientific Reports | (2025) 15:22311 3| https://doi.org/10.1038/s41598-025-06348-4 RNA extraction, reverse-transcription and quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cells with RNAiso Plus (Takara 9109) reagent and reverse-transcribed to cDNA with ALL-in-One First-Strand Synthesis MasterMix (with dsDNase). qRT-PCR was performed using SYBR GREEN (Yeasen 11201ES08) according to the manufacturer’s protocol with Article Title: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21 Article Snippet: The membranes were then developed using SuperSignalTM West Pico PLUS ECL Substrate (Thermo Fisher Scientific 34,580). .. Total RNA was extracted from cells with RNAiso Plus (Takara 9109) reagent and reverse-transcribed to cDNA with ALL-in-One First-Strand Synthesis MasterMix (with dsDNase). qRT-PCR was performed using SYBR GREEN (Yeasen 11201ES08) according to the manufacturer’s protocol with Quantitative RT-PCR:Article Title: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21. Article Snippet: The membranes were then developed using SuperSignalTM West Pico PLUS ECL Substrate (Thermo Fisher Scientific 34,580). .. 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Total RNA was extracted from cells with RNAiso Plus (Takara 9109) reagent and reverse-transcribed to cDNA with ALL-in-One First-Strand Synthesis MasterMix (with dsDNase). qRT-PCR was performed using SYBR GREEN (Yeasen 11201ES08) according to the manufacturer’s protocol with Article Title: A novel mitochondrial quality regulation gene signature for anticipating prognosis, TME, and therapeutic response in LUAD by multi-omics analysis and experimental verification Article Snippet: Synthetic complementary DNA (cDNA) was generated by mixing total RNA with a Takara PrimeScript RT reagent kit. .. The RT-qPCR technique was performed on a Article Title: S-palmitoylation of MTDH regulates ferroptosis resistance in breast cancer cell. Article Snippet: Reverse transcription PCR was performed using Hifair® AdvanceFast 1st Strand cDNA Synthesis Kit (Yeasen, 11150ES60) to obtain cDNA as template for quantitative RT-PCR. .. The quantitative RT-PCR was performed using Hieff UNICON® Advanced qPCR SYBR Master Mix (Yeasen, 11185ES03) in the SYBR Green Assay:Article Title: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21. Article Snippet: The membranes were then developed using SuperSignalTM West Pico PLUS ECL Substrate (Thermo Fisher Scientific 34,580). .. Scientific Reports | (2025) 15:22311 3| https://doi.org/10.1038/s41598-025-06348-4 RNA extraction, reverse-transcription and quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cells with RNAiso Plus (Takara 9109) reagent and reverse-transcribed to cDNA with ALL-in-One First-Strand Synthesis MasterMix (with dsDNase). qRT-PCR was performed using SYBR GREEN (Yeasen 11201ES08) according to the manufacturer’s protocol with Article Title: E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21 Article Snippet: The membranes were then developed using SuperSignalTM West Pico PLUS ECL Substrate (Thermo Fisher Scientific 34,580). .. Total RNA was extracted from cells with RNAiso Plus (Takara 9109) reagent and reverse-transcribed to cDNA with ALL-in-One First-Strand Synthesis MasterMix (with dsDNase). qRT-PCR was performed using SYBR GREEN (Yeasen 11201ES08) according to the manufacturer’s protocol with Article Title: A novel mitochondrial quality regulation gene signature for anticipating prognosis, TME, and therapeutic response in LUAD by multi-omics analysis and experimental verification Article Snippet: Synthetic complementary DNA (cDNA) was generated by mixing total RNA with a Takara PrimeScript RT reagent kit. .. The RT-qPCR technique was performed on a Amplification:Article Title: Elucidating the role of PBRM1 in NPC via RNA-seq transcriptomic sequencing. Article Snippet: Subsequently, cDNA was reverse transcribed and used for quantitative real-time PCR (qRTPCR) employing the SensiFast SYBR Kit (Bioline). .. The qRT-PCR was conducted on a |